ab_2315608 (Vector Laboratories)

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Vector Laboratories ab_2315608
Ab 2315608, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/AS-2024-1/Anti-Wheat+Germ+Agglutinin+(WGA)%2C+Unconjugated/pm42832292-71-128-132
Average 93 stars, based on 132 article reviews

ab_2315608 - by Bioz Stars, 2026-11

93/100 stars

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Immunofluorescence:

Article Title: Passive transfer of patient-derived anti-nephrin autoantibodies causes a podocytopathy with minimal change lesions
Article Snippet: .. For Immunofluorescence staining, the following antibodies were used: wheat germ agglutinin (WGA)-rhodamin (1:400; Vector-Laboratories), C3 (FITC goat anti-C3, 1:50; Cappel 55500), Collagen IV (goat anti-Collagen IV, 1:400, Southern Biotech, 1340-01). .. The following fluorochrome-conjugated secondary antibodies were used (all 1:200, Jackson ImmunoResearch Laboratories): anti-goat IgG AF488 (705-545-147), anti-goat IgG Cy3 (705-165-147), anti-rabbit IgG AF488 (711-545-152), anti-rabbit IgG Cy3 (711-165-152), anti-human IgG Cy2 (709- 225-149).

Staining:

Whole Genome Amplification:

Article Title: Anatomo-functional organization of insular networks: From sensory integration to behavioral control.
Article Snippet: Finally, FR or LYD labeling was visualized using the Vectastain ABC kit (Vector) and the Vector SG peroxidase substrate kit (SK- 4700, Vector) as a chromogen. .. For the visualization of the WGA, the sections were incubated overnight at room temperature in a primary antibody solution of goat anti-WGA (1:2000; Vector Laboratories) in 0.3 % Triton and 5 % normal rabbit serum in PBS and for 1 h in biotinylated secondary antibody (1:200; Vector Laboratories) in 0.3 % Triton and 5 % normal rabbit serum in PBS. .. Finally, in all sections the labeling was visualized using the Vectastain ABC Kit (Vector Laboratories) and then a solution of DAB (50 mg/100 ml; Sigma Millipore), 0.01 % hydrogen peroxide, 0.02 % cobalt chloride, and 0.03 % nickel ammonium sulfate in 0.1 m PB.

Article Title: Endothelial miR-34a deletion guards against aneurysm development despite endothelial dysfunction.
Article Snippet: .. 13.3, BioLegend; ATTO-550-conjugated anti-AT1R antibody, Allomone labs) or Wheat Germ Agglutinin (WGA), Rhodamine conjugate (Vector Labs) diluted in 3 % BSA. .. After that, the aortas were washed 3 times, and, if needed, incubated with the host-specific antibodies conjugated with Alexa Fluor fluorophores (dilution 1:1000; IgG H+L, Life Technologies) and DAPI (1 μg/mL, Sigma-Aldrich) for 2 h at room temperature.

Incubation:

Article Title: Nasal trigeminal projections and medullary dorsal horn neuronal activation during voluntary diving in rats
Article Snippet: .. The tissue was then incubated overnight with a goat anti-WGA primary antibody (Vector AS-2024). ..

other:

Article Title: The Nucleus of the Brachium of the Inferior Colliculus Contains Thalamic-Like and Midbrain-Like Subregions.
Article Snippet: We used mouse monoclonal antibodies for Cre recombinase (RRID: AB_2085748; MAB3120, Merck Millipore, Burlington, MA, USA), glutamic acid decarboxylase 67 (GAD67; RRID: AB_2278725; MAB5406, Merck Millipore), and parvalbumin (PV; RRID: AB_477329; P3088, Sigma ‐Aldrich); rat monoclonal antibodies for mCherry (RRID: AB_2536611; clone 16D7; Thermo Fisher Scientific, Waltham, MA, USA); rabbit polyclonal antibodies for GFP (gift from Dr. Takeshi Kaneko; RRID: AB_2921270) (Tamamaki et al. 2000 ), FG (RRID:AB_90738; AB153, Merck Millipore) and calretinin (CR; RRID: AB_2313721; AB149, Merck Millipore); guinea pig polyclonal antibodies for vesicular glutamate transporter 2 (VGLUT2; RRID: AB_2571620; MSFR106280, Frontier Institute, Ishikari, Japan) and monomeric red fluorescent protein (mRFP; gift from Dr. Takeshi Kaneko; RRID: AB_2336889) (Hioki et al. 2010 ): and goat polyclonal antibodies for CR (RRID: AB_90764; AB1550, Merck Millipore) and WGA (RRID: AB_2315608; AS ‐2024 ‐1, Vector Laboratories).

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